If inhaled if breathed in, move person into fresh air. There is a reaction that i need to use formaldehyde to synthesize a primary alcohol structure. Respiratory protection if the exposure level is exceeded, wear a full facepiece respirator equipped with a formaldehyde cartridge. Exposure controls, personal protection engineering controls use of a fume hood is recommended. Show this safety data sheet to the doctor in attendance. Beware though, that some solutions have methanol in them to stop polymerization but this could have a negative effect on your sample.
Paraformaldehyde solution 4% in pbs is commonly used to preserve tissues for routine histology in many labs. Paraformaldehyde, 4% in pbs is a readytouse fixation solution for cells or tissues. None information in this msds is from available published sources and is believed to be accurate. Formaldehyde, formalin, paraformaldehyde safe work practices.
One option is to purchase methanolfree formaldehyde aq in sealed ampoules. Commercially available formaldehyde solutions usually contain 10 15% methanol, which prevents the formaldehyde from forming the polymeric paraformaldehyde. It is electron microscopygrade paraformaldehyde dissolved in ph 7. Paraformaldehyde, 4% in pbs, readytouse fixative biotium. Material safety data sheet product name formalin solution 10 % neutral buffproduct code 28600 section 8. Store in a cool, dry, wellventilated area away from incompatible substances. Find msds or sds, a coa, data sheets and more information. Paraformaldehyde is on the right to know hazardous. Sds complies with oshas hazard communication rule 29, cfr 1910. Paraformaldehyde pfa is actually polymerized formaldehyde. Repeated or prolonged exposure to the substance can produce target organs damage. Pdf aldehydes are the most commonly used fixatives.
Methylene oxide, ac4553, ac4554, 41860, 41883 supplier. Paraformaldehyde applications applications the most important use of paraformaldehyde is as a source of formaldehyde groups in the production of many thermosetting resins, together with phenol, urea, melamine, resorcinol and other similar reagents. Sara 3 notifications must not be detached from the sds and any copying and. Formaldehyde, 10%, methanol free, ultra pure polysciences, inc. Sheet msds and the label to determine product ingredients. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Refer to certificate of analysis for lot specific data including water content. No warranty, express or implied, is made and labchem inc. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Thus, a protocol calling for 10 % formalin is roughly equivalent to 4% formaldehyde. Paraformaldehyde can be quite hazardous to handle and it is often difficult to get it to go into solution. Formaldehyde, the decomposition product of paraformaldehyde, has been listed as a carcinogen by ntp and iarc. Firstaid measures general advice if symptoms persist, call a physician. Skin contact wash off immediately with plenty of water for at least 15 minutes.
Paraformaldehyde reagentgrade,crystalline 30525894. Formaldehyde is a gas which when dissolved in water gives a formaldehyde solution. Methanol is added to slow down the polymerization to formaldehyde, which reduces the fixing power of formalin. Sigmaaldrich offers a number of paraformaldehyde products. Paraformaldehyde revision date 18jan2018 component casno weight % paraformaldehyde 30525894 90 4. In case of contact, immediately flush skin with soap and plenty of water.
Paraformaldehyde is the polymeric form of formaldehyde. Paraformaldehyde, formadehyde and formalin duke light. Formaldehyde,the decomposition product of paraformaldehyde, has been listed as a carcinogen by ntp and iarc. The user must determine suitability of this information for his application. Ultra pure, methanol free, 10 % aqueous formaldehyde is suitable for both electron and light microscopy. Formaldehyde, 20% vv aqueous solution msds msdsdigital. Protocol for making a 4% formaldehyde solution in pbs. In histology and pathology specimens preparation, usually, the fixation step is performed. Formalin can also be made in an alcoholfree form from powdered paraformaldehyde. Safety data sheet northwest missouri state university.
Pure, methanolfree formaldehyde can be made by heating the solid. Paraformaldehyde has documented uses as a disinfectant, fungicide, fixation reagent and in the preparation of formaldehyde. It is commonly used in tissue fixing and preservation, and as an organic chemical reagent. Formalin 10 % safety data sheet date of issue 26022007. Exposure was stopped during the first week for the middle and high dose groups due to high mortality. While paraformaldehyde has not been identified as a carcinogen, it should be handled with. In case of contact with eyes flush immediately with plenty of flowing water for 10 to 15 minutes. Paraformaldehyde revision date 14apr2016 general advice if symptoms persist, call a physician. In case of contact, immediately flush eyes with plenty of water for a t least 15 minutes. Collect liquid in an appropriate container or ab sorb with an. Inflammation of upper respiratory tract tissue was noted in these groups. Formaldehyde, 37% ww safety data sheet according to federal register vol. Our msds database is an easy to use, do it yourself tool, and best of all. Formaldehyde was also present at concentrations of 3.
Paraformaldehyde solution 4% in pbs cas 30525894 is rated 4. Harmful if swallowed, in contact with skin or if inhaled. A material safety data sheet has been issued describing the health, safety and. Hazards identification harmful by inhalation, in contact with skin and if swallowed. Page 1 of 10 msds formaldehyde material safety data sheet msds formaldehyde 1. Page 4 of 10 msds formaldehyde unprotected personnel from entering. I heated the paraformaldehyde to generate the formaldehyde and absorb it by dry thf, and got nothing. Paraformaldehyde solution 4% in pbs cas 30525894 scbt. Identification product name formalin, buffered, 10% phosphate buffercertified cat no.
Formalin 10% safety data sheet date of issue 26022007. Protocol for making a 4% formaldehyde solution in pbs rnsylu4 reagents required materials deionized h 2 o filter units. In fluorescence studies, paraformaldehyde has been used as as a formalin fixative to fix cells and tissues. Formaldehyde solution 37 ww, stablized with 1012% methanol. P indicates a commenced pmn substance r indicates a substance that is the subject of a section 6 risk management rule under tsca. The solution should be clear, colorless, with no precipitate and the. Sf1004, sf10020, sf100200 synonyms no information available recommended use laboratory chemicals.
Eye contact rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Formaldehyde, formalin, paraformaldehyde and glutaraldehyde. If your lab is not a regular user of formaldehyde fixatives, there are a couple of easier options that we recommend. When used in combination with glutaraldehyde, it fixes delicate tissues such as brain in vascular perfusion. Paraformaldehyde revision date 18jan2018 polymer made with any freeradical initiator regardless of the amount used. Paraformaldehyde cas 30525894 emprove essential dac find msds or sds, a coa, data sheets and more information. Limited evidence of carcinogenicity in animal studies.
Page 17 safety data sheet according to 19072006ec, article 31 printing date 19. In cell culture, a typical formaldehyde fixing procedure would involve using a 4% formaldehyde solution in phosphate buffered saline pbs on ice for 10 minutes. Page 1 of 7 paraformaldehyde cas no 30525894 material safety data sheet sds msds section 1. Use precautions against formaldehyde exposure when opening containers or entering a poorly ventilated storage area. How to generate formaldehyde from paraformaldehyde. Identification of the substancemixture and of the companyundertaking. In case of skin contact wash off with soap and plenty of water. Uses advised against food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number. The most widely used formaldehydecontaining chemical at uw is 10 % neutral buffered nb formalin, which contains 4% formaldehyde.